After this lecture, you should know:
After this lecture, you should know:
After this lecture, you should know:
After this lecture, you should know:
The steps necessary to prepare a solution to purify a protein from it: Lysing a cell, centrifuging out the cell debris, salting out, dialysis
The three types of column chromatographic methods necessary to purify a protein. What you must consider for each method, how you elute the protein in each method
MP3 audio file of the second half of Chapter 3 from 2 September
After this lecture, you should know:
What SDS-PAGE is and what you can learn from a SDS-PAGE gel
Antibodies: What an Fc and the Fab are
What ELISA stands for, the two types of ELISA techniques and the best use for each type
What a Western blot is and what you can learn from one
What a 2D-gel is and waht you can learn from a two-dimensional gel experiment
What MALDI-TOF means, the basics of the instrument and what you can learn from a MALDU-TOF mass spectrogram
After this lecture, you should know:
The coordination state of Fe+2 and what a porphyrin ring is
How myoglobin and hemoglobin bind the porphyrin ring and what the proximal and distal histidines do
The roles of myoglobin in organisms
The role of hemoglobin in organisms
The difference between myoglobin and hemoglobin
The evolutionary relationshiip between myoglobin and hemoglobin
What divergent and convergent evolution mean
After this lecture, you should know:
The relationship between Kd, the pO2 values necessary to give fractional saturation values of 50% for myoglobin and hemoglobin and how that directly relates to the function of each protein.
Explain the fractional saturation curve knowing the arterial and venous pO2 values and how similar they are to the p50 values of each protein
What cooperativity is, what is required to exhibit cooperative behaviour and the two models of cooperativity
The basis of cooperativity in hemoglobin with EXACT chemical changes occurring in the subunits as oxygen binds
Allosteric effectors of hemoglobin (and understanding that allostery and cooperativity are different things)
After this lecture, you should know:
Zoom recording of 19 September Lecture on Chapter 8 Part 2(NOTE: You will have to download the file while on campus in before you can play it, it will not stream from the web server)
After this lecture, you should know:
After these lectures, you should know:
After this lecture, you should know:
After this lecture, you should know: