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CHEM525: Biochemistry Laboratory Techniques

Course Prelabs:

The following prelab exercises must be completed, brought to class and turned in at the start of the indicated laboratory session. Faiure to turn in the prelab will result in an automatic letter grade reduction in that week's lab report.

Please consult the notes associated with the prelabs for tips and things to consider when preparing for the week's work.

Prelab 1: Practical Bioinformatics: Looking AND Seeing with Entrez / BLAST and Chimera

Prelab 2: Cell Lysis

Prelab 3: FPLC and Ion Exchange Chromatography

Prelab 4: SDS-PAGE analysis of FPLC fractions

Prelab 5: Analysis of Polymer Depolymerization Kinetics by reversed phase HPLC

Prelab 6: Influence of [Substrate] on enzymatic reactions

Prelab 7: Influence of pH on enzymatic reactions

Prelab 8: Chemical Denaturation of Proteins

Prelab 9: MALDI-TOF MS analysis of purified FPLC fractions