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CONTACT INFORMATION
Dr. Jason C. Hurlbert
301B Sims Science Bldg
Rock Hill, SC 29733, USA
803/323-4928
803/323-2246 (fax)

CHEM525: Biochemistry Laboratory Techniques

Experiment Lectures

Week of Topic / Procedure
Monday, Aug 24 No Class Meeting
Aug 31 Course Overview and Semester Plan
Sept 7 No Class Meeting
Sept 14

1) Practical Bioinformatics: Looking AND Seeing

Sept 21

2) Protein Expression and Purification: Cell Lysis
Sept 28

3) Protein Expression and Purification:Ion Exchange Chromatography
Oct 5

4) Analysis of Protein Purification: SDS-PAGE and Western Blot
Oct 12

5) Analysis of Depolymerization Products by HPLC
Oct 19 No Class Meeting - Fall Break
Oct 26

6) Kinetics by Spectrophotometry I: Influence of [Substrate] on enzymatic reactions
Nov 2

7) Kinetics by Spectrophotometry II: Influence of pH on enzymatic reactions
Nov 9 8) Kinetics by Spectrophotometry III: Chemical Denaturation of Proteins
Nov 16

9) Mass Spectrometry: Biological Mass Spectrometry

  
Nov 30

Thanksgiving Break

Prelab Session Will Meet
Lab Sections Will Not Meet
Dec 7

Final Exam

(FLABB = Fundamental Laboratory Approaches for Biochemistry and Biotechnology)