Winthrop.edu
Dr. Jason C. Hurlbert, 301B Sims Science Building , Rock Hill, SC  29733  •  803/323-4928  •  803/323-2246 (Fax)   
Hurlbert Lab Home
Useful Programs
Questions?
 
 

Protocols and Operating Procedures

Links to the protocols and standard operating procedures used in the lab are below. As usual, trust your eyes and hands before blindly following a protocol. Check the reagents we have in stock and be certain that everything matches.

Intrumentation

Voyager DE-STR MALDI-TOF mass spectrometer

Beckman DU-800 Spectrophotometer

Any departmental Centrifuge

Biological Safety Cabinet

Fisher Scientific Accumet AB15 Calibration

 

Protocols

Alkaline Lysis Miniprep

Calcium Competent Cell Production

Bacterial transformation (Calcium competent cells)

Cell culture

SDS-PAGE

Western Blotting (Semi-dry)

Sample Cleanup with Zip Tips prior to MALDI-TOF MS analysis

Optimizing MALDI-TOF Mass Spectra

Post-source decay experiments on the MALDI-TOF

In-gel trypsin digestion and analysis by MALDI-TOF

 

 

 

 

 

 

 

 

 

 

 

Rock Hill, South Carolina   29733
Copyright © 2005 - 2007 Winthrop University
University Disclaimer Statement