Week of |
Topic / Procedure |
Aug 19 |
Course Overview and Semester Plan |
Aug 26 |
1) Practical Bioinformatics: Looking AND Seeing |
Sept 2 |
2) Spectrophotometric Assays |
Sept 9 |
3) Protein Expression and Purification:
Experiment 3a: Cell Lysis |
Sept 16 |
3) Protein Expression and Purification:
Experiment 3b: FPLC: Ion Exchange Chromatography |
Sept 23 |
4) Analysis of Protein Purification: SDS-PAGE and Western Blot |
Sept 30 |
5) Analysis of Depolymerization Products by HPLC |
Oct 7 |
6) Kinetics by Spectrophotometry I: Influence of [Substrate] on enzymatic reactions |
Oct 14 |
No Class |
Oct 21 |
7) Kinetics by Spectrophotometry II: Influence of pH on enzymatic reactions |
Oct 28 |
8)Kinetics by Spectrophotometry III: Chemical Denaturation of Proteins |
Nov 4 |
|
Nov 11 |
9) Mass Spectrometry I: MALDI-TOF MS analysis of purified FPLC fractions |
Nov 18 |
Final Report Preparation |
Nov 25 |
Thanksgiving Break
Prelab Session Will Meet
Lab Sections Will Not Meet |
Dec 2 |
Final Exam |
Dec 9 |
Final Lab Report Due by 3 PM |