Experiment Lectures
1) Practical Bioinformatics: Looking AND Seeing
2) Spectrophotometric Assays
3) Protein Expression and Purification:
Experiment 3a: Cell Lysis
Experiment 3b: FPLC: Ion Exchange Chromatography
4) Analysis of Protein Purification: SDS-PAGE and Western Blot
5) Analysis of Depolymerization Products by HPLC
6) Kinetics by Spectrophotometry I: Influence of [Substrate] on enzymatic reactions
7) Kinetics by Spectrophotometry II: Influence of pH on enzymatic reactions
Final Report Preparation
Thanksgiving Break
Prelab Session Will Meet Lab Sections Will Not Meet
(FLABB = Fundamental Laboratory Approaches for Biochemistry and Biotechnology)