SPE: Spectrophotometric Determination of Copper

Part A: Spectrum of Copper-Ammonia Complex

Wavelength

(nm)

% T

Absorbance

Wavelength

(nm)

% T

Absorbance

400

 

 

540

 

 

420

 

 

560

 

 

440

 

 

580

 

 

460

 

 

600

 

 

480

 

 

620

 

 

500

 

 

640

 

 

520

 

 

660

 

 

Wavelength with maximum absorbance (l max):___________________

 

Part B: Preparation of Copper Standards

1.      Copper Standard Concentration:______________________

2.      Diluted Copper Standards:

Standard Number

1

2

3

4

5

Prepared by

 

 

 

 

 

Volume pipetted, mL

 

 

 

 

 

Concentration, M*

 

 

 

 

 

 %T

 

 

 

 

 

Absorbance

 

 

 

 

 

*Show 1 calculation of diluted standard concentration: