Principal Investigator/Program Director (Last, First, Middle):

Parker, Chasta L.                                 

 

BIOGRAPHICAL SKETCH

Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2.
Follow this format for each person.  DO NOT EXCEED FOUR PAGES.

 

NAME

Chasta L. Parker

POSITION TITLE

 

Associate Professor of Chemistry

 

eRA COMMONS USER NAME

 

EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)

INSTITUTION AND LOCATION

DEGREE

(if applicable)

YEAR(s)

FIELD OF STUDY

Salisbury University, Salisbury, MD

B.S.

1992 – 96

Chemistry

University of South Carolina, Columbia, SC

Ph.D.

1996 – 01

Biochemistry

 

A.      Positions and Honors.

 

Positions

1996 – 2001          Graduate Research Assistant, University of South Carolina, Columbia, SC

2001 – 2007          Assistant Professor of Chemistry, Winthrop University, Rock Hill, SC

2007 – Present     Associate Professor of Chemistry, Winthrop University, Rock Hill, SC

           

Professional Memberships

1996 – present      Member, American Chemical Society

1997 – present      Member, Sigma Xi Scientific Research Society

1997 – 1998          President, Society for the Advancement of Chemical Sciences

2001 – present      Member, Council on Undergraduate Research

2001 – present      Member, South Carolina Academy of Sciences

2004 – present      Member, American Society for Biochemistry and Molecular Biology

2006 – present      Member, American Association for the Advancement of Science

2007 – 2008          Councillor, South Carolina Academy of Sciences

2008 – present      Secretary, South Carolina Academy of Sciences

 

Honors

1998                      Joseph W. Bouknight Graduate Teaching Award, University of South Carolina

1998                      Distinguished Teaching Assistantship, University of South Carolina

2003                      Presidential Citation in Recognition for Earning a competitive research grant, Winthrop University

2005                      2005 American Society for Biochemistry and Molecular Biology Undergraduate Faculty Travel Award to annual meeting in San Diego, CA. 

2005                      Presidential Citation in Recognition for Acting as Winthrop’s Liaison to Host the South Carolina Academy of Science meeting at Winthrop University.

2006                      2006 American Society for Biochemistry and Molecular Biology Undergraduate Faculty Travel Award to annual meeting in San Francisco, CA.

2006                      Presidential Citation in Recognition for Earning the NIH-INBRE grant, Winthrop University.

2007                      Elected to a council position for the South Carolina Academy of Sciences.

2007                      Awarded Tenure and promotion at Winthrop University.

2008                      Elected Secretary for the South Carolina Academy of Sciences.

 

Courses Taught

Chem 105             General Chemistry I

Chem 107             General Chemistry Laboratory I

Chem 108             General Chemistry Laboratory II

Chem 351             Independent Study

Chem 521             Nutritional Biochemistry

Chem 523             Biochemistry I

Chem 524             Biochemistry II

Chem 525             Biochemical Techniques Laboratory

Chem 551/552      Research I and II

Biol 471/472          Undergraduate Research in Biology

Hmxp 102              The Human Experience:  Who Am I?

 

B.      Peer-reviewed Publications and Presentations

 

Publications

1.      Schreck, S.F., Parker, C., Plumb, M.E. and Sodetz, J.M. (2000) Human Complement Component C8g.  Biochim. Biophys. Acta  1482, 199-208.

2.      Ortlund, E., Parker, C., Schreck, S.F., Sodetz, J.M. and Lebioda, L.  (2002) Crystal Structure of Human Complement Protein C8g at 1.2Å Resolution Reveals a Lipocalin-like Fold and a Distinct Ligand Binding Site.  Biochemistry.  41(22), 7030-7037.

3.      Parker, C. and Sodetz, J.M. (2002) Role of the Human C8 Subunits in Complement-Mediated Bacterial Killing:  Evidence that C8g is not Essential.  Mol. Immunol.  39(7-8), 453-458.

4.      Parker, C. (2007) Focus on Our Future: Student Zainab Ghadiyali.  Enzymatic 4(3), 8-10.

 

Presentations (bold indicates undergraduate student)

1.      Schreck, S.F., Parker, C., Lebioda, L. and Sodetz, J.M. (1998) “Production and Characterization of Recombinant Human Complement Component C8g,”.  Bull. S.C. Acad. Sci.  60, 106. Presented as a poster.

2.      Schreck, S.F., Parker, C., Lebioda, L. and Sodetz, J.M.  (1998) “Production and Characterization of Recombinant Human Complement Component C8g”, FASEB J. 12, A293. Presented as a talk at ASBMB meeting.

3.      Ortlund, E., Parker, C., Schreck, S.F., Minor, W., Sodetz, J.M., and Lebioda, L. (2001) “Three

            Dimensional X-ray Crystal Structure of Human Complement Protein C8 Gamma at 1.2A

            Resolution”, Tenth South Carolina Statewide Research Conference on Molecular Approaches to Problems in Biology, Isle of Palms, S.C.  Presented as a poster.

4.      Parker, C., Ortlund, E., Schreck, S.F., Lebioda, L. and Sodetz, J.M. (2001) “Human Complement

            Component C8: Structure-Function Analysis of the C8g Subunit”,  Bull. S.C. Acad. Sci. 74, 94. SCAS, Coastal Carolina University, Conway, S.C. presented as a talk.

5.      Ortlund, E., Parker, C., Schreck, S.F., Minor, W., Sodetz, J.M., and Lebioda, L. (2001)  “Three

            Dimensional Crystal Structure of Human Complement Protein C8 Gamma at 1.2 A Resolution

            Suggests a Mechanism for Signaling Between the C8 Alpha and C8 Gamma Subunits”, Mid-

            Atlantic Crystallography Conference, Williamsburg, V.A. Presented as a talk.

6.      Sodetz, J.M., Ortlund, E., Parker, C.L., Schreck, S.F., and Lebioda, L.  (2002)  “The Crystal Structure of Human Complement Protein C8g Provides New Insight into its Function.”  XIX International Complement Workshop, Palermo, Italy. Presented as a poster.

7.      Sodetz, J.M., Ortlund, E., Parker, C.L., and Lebioda, L. (2003) Human Complement Protein C8g:  The Sole Lipocalin in the Complement System.  International Lipocalin Meeting, Copenhagen, Denmark.  August 2003, Poster.

8.      Bork, D., Murphy, K., Chiswell, B., Sodetz, J.M., and Parker, C.L. (2003) “Production of a Recombinant C8a Protein Fragment for Structural Analysis.”  Bull. S.C. Acad. Sci. 76, 61. SCAS meeting at Clemson University, Clemson, S.C.  Presented as a talk.

9.      Rai, N., Bork, D., Murphy, K., Dimond, E., Chiswell, B., Sodetz, J.M., and Parker, C.L. (2003) “Production of a Recombinant C8a Protein Fragment for Structural Analysis.”  Poster presentation at Duke University’s Duke Excellence in Chemical Biology Undergraduate Poster Contest.

10.  Dimond, E., Bork, D., Murphy, K, Rai, N., Chiswell, B., Sodetz, J.M., and Parker, C.L. (2003)  “Production of a Recombinant C8a Protein Fragment for Structural Analysis.”  55th Southeast Regional Meeting of the American Chemical Society. presented as a poster. Atlanta, GA.

11.  Walker, L., and Parker, C. (2005) “Expression and Purification of Recombinant AdipoR1 in Sf9 cells” Poster Presentation. 19th National Conference on Undergraduate Research. Lexington, VA (VMI) April 21-23.

12.  Fowlkes, V., and  Parker, C. (2005) “Reproduction of Recombinant MACPF C8alpha in Sf9 Insect Cells” Oral Presentation. 19th National Conference on Undergraduate Research. Lexington, VA (VMI) April 21-23.

13.  Parker C., Bork, D., Dimond, E., Rai, N., Murphy, K., Chiswell, B., and Sodetz, J. (2004) “Production of a recombinant membrane-binding region of C8α for structural analysis.”  A81.6. Presented as a poster at ASBMB in Boston, MA.  July 12 -16.

14.  Walker, L., and Parker, C.  (2005) “Expression and Purification of AdipoR1 in Sf9 cells.” FASEB J.  19(5), A234.1. Presented as a Poster at ASBMB meeting in San Diego, CA. April 2-6.

15.  Fowlkes, V., and Parker, C. (2005) “Cloning and Expression of C8α in Sf9 insect cells.” FASEB J.  19(5), A234.5. Presented as a poster at ASBMB meeting in San Diego, CA. April 2-6.

16.  Parker, C.  (2005) “The Biochemistry Laboratory:  Using an Integrative Approach to Teaching Basic Biochemical Techniques.” FASEB J.  19(5), A800.19. Presented as a poster at ASBMB meeting in San Diego, CA. April 2-6.

17.  Walker, L., and Parker, C. (2005) “Expression and Purification of Recombinant AdipoR1 in Sf9 cells.” Bull. S.C. Acad. Sci.  Volume LXVII. SCAS meeting at Winthrop University March 16th. Presented as a talk.

18.  Parker, C.  (2006) “The Biochemistry Laboratory: Using an Integrative Approach to Engage Students in Deep Learning.” FASEB J.  20(5), A975. Presented as a poster at ASBMB meeting in San Francisco, CA. April 1 – 5.

19.  Ghadiyali, Z., and Parker, C. (2006) “Production of the MACPF Region of Complement C8alpha in Sf9 Insect Cells.”  FASEB J. 20(4), A462 – A463. Presented as a poster at ASBMB meeting in San Francisco, CA. April 1 – 5.

20.  Ghadiyali, Z., and Parker, C. (2006) “Production of Recombinant MACPF Complement C8α in Sf9 Insect Cells for Protein Production and Purification.” Presented as a poster at the National Conference on Undergraduate Research in Asheville, NC April 6-8.

1.      Glazener, R., and Parker, C. (2006) “Kinetic Characterization of Arylamine N-Acetyltransferase 2 Using the Substrate Sulfamethazine.” Presented as a poster at the 1st Annual South Carolina Chemistry Colloquium in Columbia, SC  October 7.

2.      Kissinger, S., and Parker, C. (2006) “Expression of Adiponectin Receptor 1 in Sf9 Cells.” Presented as a poster at the 1st Annual South Carolina Chemistry Colloquium in Columbia, SC  October 7.

3.      Ghadiyali, Z.,  and Parker, C. (2006) “Expression and purification of Human Adiponectin receptor, AdipoR2 in Sf-9 Insect for Structural Analysis and Biochemical Assays.” Presented as a talk at the Southeast Regional Meeting of the National Organization of Black Chemists and Chemical Engineers, Augusta, GA. November 10 -11.

4.      Baker, I., and Parker, C. (2006) “Cloning, Expression and Purification of Arylamine N-Acetyltransferase 2” Presented as a poster at the Southeast Regional Meeting of the National Organization of Black Chemists and Chemical Engineers, Augusta, GA. November 10 -11.

5.      Parker, C., Ghadiyali, Z., Kissinger, S., McKinney, K., and Hurlbert, J. “ Expression and Purification of Human Adiponectin Membrane Receptors, AdipoR1 and AdipoR2” (2007) Experimental Biology, Washington DC, April 28 – May 2.

6.      Glazener, R., Baker, I., Hurlbert, J, and Parker, C. (2007) “Kinetic characterization of Arylamine N-Acetyltransferase 2 using the substrates sulfamethazine and 5-aminosalicylic acid” National Meeting of the American Chemical Society, Chicago, IL, March 25-29.

7.      Baker, I., and Parker, C. (2007) “Expression and Purification of Human Arylamine N-Acetyltransferase 2” National Meeting of the American Chemical Society, Chicago, IL, March 25-29.

8.      Ghadiyali, Z., and Parker, C. (2007) “Cloning, Expression and Purification of Human Adiponectin Receptor, AdipoR2” National Meeting of the American Chemical Society, Chicago,IL, March 25-29.

9.      Kissinger, S., Walker, L., and Parker, C. (2007) “Expression of Adiponectin Membrane Receptor 1 in Sf9 cells” National Meeting of the American Chemical Society, Chicago,IL, March 25-29.

10.  Glazener, R., Baker, I., Hurlbert, J, and Parker, C. (2007) “Kinetic characterization of Arylamine N-Acetyltransferase 2” Submitted: National Conference on Undergraduate Research, CA, April 12 – 14.

11.  Kissinger, S., Walker, L., Hurlbert, J. and Parker, C. (2007) “Expression of Human Adiponectin Membrane Receptor 1 in Sf9 cells” National Conference on Undergraduate Research, CA, April 12 – 14.

12.  Ghadiyali, Z., and Parker, C. (2007) “Cloning, Expression, and Purification of Human Adiponectin Receptor, AdipoR2 for Structural Analysis and Biochemical Assays”  National Organization of Black Chemists and Chemical Engineers, Fl, April 1-7.

13.  Faile, K., James, A., and Parker, C. (2007) “Expression of Adiponectin Protein Receptors: AdipoR1 and AdipoR2”  USC 2nd Annual Chemistry Coloquium, Columbia, SC, Oct. 6.

14.  James, A., Faile, K.L., and Parker, C. (2008) "Adiponectin Membrane Receptors: ADIPOR1 and ADIPOR2"  Oral presentation at the South Carolina Academy of Sciences, Clemson, SC, March 20th. (sigma xi and AAAS award).

15.  Faile, K., James, A., and Parker, C. (2008) “Adiponectin Membrane Receptors 1 and 2" Poster presented at Experimental Biology - American Association of Biochemistry and Molecular Biology, San Diego, CA, April 4 - 8th.

16.  James, A., Faile, K.L., and Parker, C. (2008) "Adiponectin Membrane Receptors: ADIPOR1 and ADIPOR2"  Oral presentation at the National Conference on Undergraduate Research, Salisbury, MD, April 10 - 12th.

 

A.      Research Support.

 

Ongoing Research Support:  

2 P20 RR016461-05A1                 Baynes (PI)                                         07/01/2005 – 04/30/2010

SC IdeA Network of Biomedical Research Excellence

The major goal at Winthrop University is to increase research capacity through faculty development, research infrastructure development, and increased student research participation particularly those from underrepresented groups.

Role: Co-PI Project Investigator for Winthrop University

 

CHE-0722722                               Hurlbert (PI)                                         08/15/2007 – 07/31/2010

National Science Foundation – Major Research Instrumentation Program

This grant was awarded to provide a Voyager DE STR MALDI TOF and An AB/Sciex API 2000 Triple Quad MS/MS instruments for research and teaching at Winthrop University.

Role: Co-PI

 

5-29183                                         Parker (PI)                                           05/16/2007 – 06/30/2008

Winthrop University Research Council

The major goal of this grant is to provide funds for student summer/academic year salary.

Role: PI

 

Completed Research Support:  

529145                                                                  Parker (PI)                   05/16/2006 – 06/30/2007

Winthrop University Research Council                                                   

Production and Purification of Recombinant Human Adiponectin Membrane Receptor 1 (AdipoR1) form Sf 9 insect cells

This grant funded a student co-investigator for summer research to work on the production and purification of AdipoR1 for structural studies.

Role: PI

 

529154                                                                  Parker (PI)                   05/16/2006 – 06/30/2007

Winthrop University Research Council                                                   

Development of an Enzyme Kinetic Assay to Measure the Rate of Reaction of Pigeon Liver N-Acetyltransferase 2 (NAT 2)

The purpose of this grant was to fund a student co-investigator for the summer to work on adapting a useable assay for analysis of NAT 2 activity.  The grant also supplied some money for supplies.

Role: PI

 

529108                                                                  Lammi (PI)                  05/16/2005 – 06/30/2006

Winthrop University Research Council                                                   

Flourescent Monitoring of Metal-Induced Conformational Change in Amyloid Beta Peptide

The purpose of this grant was to fund an undergraduate research student in the summer 2005 and to   purchase required supplies.

Role: Co - PI

 

529071                                                                  Parker (PI)                   05/16/2004 – 06/30/2005

Winthrop University Research Council   

Purification, Crystallization and Structural Analysis of the Membrane Binding Domain of Human Complement Protein C8alpha.

This grant was used to fund an undergraduate student to work on the project over summer 2004.

Role: PI

 

NSF/EPSCoR EPS-0132573 and NIH/BRIN 8-P0RR16461A  Parker (PI)  10/10/2003 – 04/30/2004

Winthrop University SC-BRIN/EPSCoR Network Seminar Program

This grant is being used to fund speakers from all over South Carolina to speak at the Winthrop University Department of Chemistry seminar series.

Role: PI

 

NSF/EPSCoR EPS-0132573 and NIH/BRIN 8-P0RR16461A  Sodetz (PI)  05/15/2002 – 08/30/2003

SC-BRIN/EPSCoR

Structural Analysis of the Membrane-Binding Domain of Human Complement Protein C8a

The goal of this project was to create cDNA clones of the MACPF region of C8a, express it in bacteria and attempt crystallization.

Role:  Co-PI

 

NSF/EPSCoR EPS-0132573 and NIH/BRIN 8-P0RR16461A  Sodetz (PI)  09/01/2003 – 06/30/2004

SC-BRIN/EPSCoR

Structural Analysis of the Membrane-Binding Domain of Human Complement Protein C8a

The goal of this project was to use cDNA clones of the MACPF region of C8a to increase expression levels in bacteria and attempt crystallization.

Role:  Co-PI

 

529010                                                                              Parker (PI)       05/1/2003 – 06/30/2004

Winthrop University Research Council   

Structural Analysis of the Membrane-Binding Domain of Human Complement Protein C8 Alpha

The purpose of this grant was to fund two undergraduate research students in the summer 2003.

Role: PI