General Chemistry II Course
Competencies
Lesson 8: Enzymes
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Chemical kinetics
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Understand what is meant by the rate law and outline the experimental procedures
and methodology to determine reaction order
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Use a given rate law to calculate rate constants and their associated units
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Understand how to experimentally determine a reaction's activation energy
using Arrhenius plots
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Be able to use activiation energies to predict rate constants at different
temperatures
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Understand the effect of activation energy on the degree of rate constant
changes with temperature
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Michaelis-Menton kinetics
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Write equilibria associated with enzyme-substrate interactions
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Understand how reaction order changes with substrate concentration
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Be able to use the Lineweaver-Burke relationship to calculate Vmax,
turnover number, and KM
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Understand the significance of KM; understand the relation of
KM to enzyme-substrate complex stability and to maximum reaction
rate
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Enzyme-substrate interactions
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Be able to draw and to clearly explain reaction energy diagrams for enzyme-substrate
interactions
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Understand the effects of inhibitors and what is specifically meant by
IC50
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Understand and clearly explain the basis for important types of noncovalent
enzyme-substrate interactions
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Clearly describe the enzyme inhibition process