Processing 1 helix: Transmembrane helix 1 from 88 (in) to 103 (out) : -2.111 Score = -0.002111 Processing 1 helix: Transmembrane helix 1 from 85 (out) to 100 (in) : -7.761 Score = -0.007761 Processing 9 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -64.702 Transmembrane helix 2 from 23 (out) to 38 (in) : -285.098 Transmembrane helix 3 from 54 (in) to 69 (out) : -986.537 Transmembrane helix 4 from 73 (out) to 88 (in) : -279.605 Transmembrane helix 5 from 92 (in) to 107 (out) : -48.968 Transmembrane helix 6 from 111 (out) to 126 (in) : -565.143 Transmembrane helix 7 from 130 (in) to 145 (out) : -541.302 Transmembrane helix 8 from 149 (out) to 164 (in) : -226.431 Transmembrane helix 9 from 168 (in) to 183 (out) : -48.5 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -64.198 Transmembrane helix 2 from 23 (in) to 38 (out) : -279.002 Transmembrane helix 3 from 66 (out) to 81 (in) : -568.393 Transmembrane helix 4 from 85 (in) to 100 (out) : -6.439 Transmembrane helix 5 from 104 (out) to 119 (in) : -494.955 Transmembrane helix 6 from 123 (in) to 138 (out) : -252.438 Transmembrane helix 7 from 149 (out) to 164 (in) : -226.431 Transmembrane helix 8 from 168 (in) to 183 (out) : -48.5 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -64.702 Transmembrane helix 2 from 23 (out) to 38 (in) : -285.098 Transmembrane helix 3 from 78 (in) to 93 (out) : -105.96 Transmembrane helix 4 from 97 (out) to 112 (in) : -202.168 Transmembrane helix 5 from 123 (in) to 138 (out) : -252.438 Transmembrane helix 6 from 149 (out) to 164 (in) : -226.431 Transmembrane helix 7 from 168 (in) to 183 (out) : -48.5 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -64.198 Transmembrane helix 2 from 23 (in) to 38 (out) : -279.002 Transmembrane helix 3 from 77 (out) to 92 (in) : -118.959 Transmembrane helix 4 from 96 (in) to 111 (out) : -159.428 Transmembrane helix 5 from 122 (out) to 137 (in) : -243.293 Transmembrane helix 6 from 164 (in) to 179 (out) : -20.268 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -64.702 Transmembrane helix 2 from 23 (out) to 38 (in) : -285.098 Transmembrane helix 3 from 88 (in) to 103 (out) : -2.111 Transmembrane helix 4 from 122 (out) to 137 (in) : -243.293 Transmembrane helix 5 from 164 (in) to 179 (out) : -20.268 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -48.003 Transmembrane helix 2 from 88 (in) to 103 (out) : -2.111 Transmembrane helix 3 from 122 (out) to 137 (in) : -243.293 Transmembrane helix 4 from 164 (in) to 179 (out) : -20.268 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 11 (in) to 26 (out) : -55.045 Transmembrane helix 2 from 88 (out) to 103 (in) : -11.089 Transmembrane helix 3 from 164 (in) to 179 (out) : -20.268 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 88 (out) to 103 (in) : -11.089 Transmembrane helix 2 from 164 (in) to 179 (out) : -20.268 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -64.198 Transmembrane helix 2 from 23 (in) to 38 (out) : -279.002 Transmembrane helix 3 from 52 (out) to 67 (in) : -1033.02 Transmembrane helix 4 from 71 (in) to 86 (out) : -374.045 Transmembrane helix 5 from 90 (out) to 105 (in) : -26.326 Transmembrane helix 6 from 109 (in) to 124 (out) : -596.899 Transmembrane helix 7 from 128 (out) to 143 (in) : -408.487 Transmembrane helix 8 from 149 (in) to 164 (out) : -219.269 Transmembrane helix 9 from 168 (out) to 183 (in) : -55 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -64.702 Transmembrane helix 2 from 23 (out) to 38 (in) : -285.098 Transmembrane helix 3 from 65 (in) to 80 (out) : -607.24 Transmembrane helix 4 from 84 (out) to 99 (in) : -11.387 Transmembrane helix 5 from 103 (in) to 118 (out) : -448.601 Transmembrane helix 6 from 122 (out) to 137 (in) : -243.293 Transmembrane helix 7 from 149 (in) to 164 (out) : -219.269 Transmembrane helix 8 from 168 (out) to 183 (in) : -55 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -64.198 Transmembrane helix 2 from 23 (in) to 38 (out) : -279.002 Transmembrane helix 3 from 77 (out) to 92 (in) : -118.959 Transmembrane helix 4 from 96 (in) to 111 (out) : -159.428 Transmembrane helix 5 from 122 (out) to 137 (in) : -243.293 Transmembrane helix 6 from 149 (in) to 164 (out) : -219.269 Transmembrane helix 7 from 168 (out) to 183 (in) : -55 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -64.702 Transmembrane helix 2 from 23 (out) to 38 (in) : -285.098 Transmembrane helix 3 from 88 (in) to 103 (out) : -2.111 Transmembrane helix 4 from 122 (out) to 137 (in) : -243.293 Transmembrane helix 5 from 149 (in) to 164 (out) : -219.269 Transmembrane helix 6 from 168 (out) to 183 (in) : -55 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -48.003 Transmembrane helix 2 from 88 (in) to 103 (out) : -2.111 Transmembrane helix 3 from 122 (out) to 137 (in) : -243.293 Transmembrane helix 4 from 149 (in) to 164 (out) : -219.269 Transmembrane helix 5 from 168 (out) to 183 (in) : -55 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -56.035 Transmembrane helix 2 from 87 (out) to 102 (in) : -9.125 Transmembrane helix 3 from 149 (in) to 164 (out) : -219.269 Transmembrane helix 4 from 168 (out) to 183 (in) : -55 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 11 (out) to 26 (in) : -47.755 Transmembrane helix 2 from 87 (in) to 102 (out) : -3.175 Transmembrane helix 3 from 164 (out) to 179 (in) : -22.132 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 87 (in) to 102 (out) : -3.175 Transmembrane helix 2 from 164 (out) to 179 (in) : -22.132 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.002111 1 helix (-) : Score = -0.007761 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 88-103 Re-entrant helices: Not detected. Helix count: 1 N-terminal: in Score: -0.002111