Prelab Lecture Lab Meeting Lecture Topic Lab Due at beginning of class
  11-Jan   Bioinformatics -  computer lab  
15-Jan   Introduction to Lab and Biochemistry Lab Techniques   Bioinformatics HW
  18-Jan MLK Day - No Lab  
22-Jan   Overview of Molecular Cloning    
  25-Jan   PCR, Miniprep, and Digestion  
29-Jan   Working with Polynucleic Acids    
  1-Feb   Purification/Ligation  
5-Feb   Visualization of Biomolecules   Cloning Introduction
  8-Feb   In Lab:  Transformation and media prep  
Computer Lab: Visualization
12-Feb   DNA Sequencing In-lab:  Colony selection and Inoculation  
  15-Feb   PCR Screen, Column Purification, Transformation Visualization HW
19-Feb   Bacteria Strain Specialization  
  22-Feb   Preparation for Protein Purification (Buffers, Gels, Media)  
26-Feb   Lecture: Protein Purification 1  
  29-Feb   Lysis and Chromatography Cloning Formal Report
4-Mar   Electrophoresis    
  7-Mar   Electrophoresis, Concentration, and Activity Assay  
12-Mar   Quantitation Methods    
  14-Mar Spring Break  
18-Mar  
  21-Mar   Computer Lab - Advanced Chimera  
25-Mar   Enzyme Activity Analysis  
  28-Mar   Enzyme Kinetics
1-Apr   pH and Activity   Purification Formal Report
  4-Apr   pH Dependence of Activity  
8-Apr   Structure and Activity    
  11-Apr   Denaturation and Activity  
15-Apr   Inhibition   Introduction and Methods of Kinetics Analysis
  18-Apr   Inhibitors and Activity  
22-Apr   Exam    
  25-Apr   Lab Practical Final Paper Due

Syllabus

Lectures

Prelab

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